BSL-2 Virology-Based Assays
Innovative virology research with safety and precision. At Vaxonyx LLC, we perform a wide range of BSL-2 level virology assays to support antiviral screening, vaccine research, and molecular pathogenesis studies. Our facility and expertise enable high-quality in vitro virology studies using established viral systems under biosafety level 2 (BSL-2) containment.
Virus Culture and Quantification
- Virus propagation in mammalian and insect cell lines
- Plaque assay, TCID₅₀, and focus-forming assays
- Viral load quantification by qPCR and immunodetection methods
- Determination of infectious titer and replication kinetics
Antiviral Efficacy and Cytotoxicity Testing
- In vitro antiviral compound screening and dose–response analysis
- Determination of EC₅₀, CC₅₀, and Selectivity Index (SI)
- Time-of-addition and mechanism-of-action studies
- Cytotoxicity and cell viability assays (MTT, LDH, ATP-based)
Immunological and Molecular Virology Assays
- ELISA for viral antigen or antibody detection
- Immunofluorescence and immunocytochemistry
- Flow cytometry-based infection quantification
- qRT-PCR for viral RNA and host response genes
Recombinant and Pseudovirus Systems
- Development and testing of pseudotyped virus models
- Safe surrogate systems for studying high-risk viral entry pathways
- Evaluation of vaccine candidates, neutralizing antibodies, and entry inhibitors
Data Analysis and Reporting
- Statistical analysis of viral growth and inhibition curves (GraphPad Prism, R, Python)
- Interpretation of antiviral and immunological response data
- Custom visual data reports and summaries for publication or regulatory use
Applications
- Vaccine antigen discovery and characterization
- Antiviral drug screening and mechanistic studies
- Host-pathogen interaction and immune response profiling
- Support for IND-enabling preclinical studies